![]() ![]() Kit contents accommodate 32 students (8 4-student workstations) Primary antibody (monoclonal mouse anti-myosin light chain), lyophilized Secondary antibody (polyclonal goat anti-mouse antibody conjugated to myosin peroxidase horseradish (HRP), freeze-dried HRP A color detection reagent HRP B color detection reagent 10x Tris-glycine, 1 L Non-fat dry milk blocker 10x phosphate buffered saline (PBS), 2 x 100 ml Tween 20 10%, 5 ml Nitrocellulose sheets, 0.45 µm (8) Blotting paper sheets (16) Reagent tubes (25) Curriculum including teacher guide, student manual and graphic guide quick. Triune Solutions Inc - Offering Bio-Rad Vertical Mini Trans-blot Electrophoretic Transfer Cell, For Gel Electrophoresis, Model Name/Number: 1703930 at Rs. Nuclear extracts for immunoprecipitation and Western blot analyses of 3E-H. Follow Us Bio-rad LinkedIn Bio-rad Antibodies LinkedIn Bio-rad. Directed Mutagenesis Kit (Agilent Genomics) according to manufacturer. Minigel apparatus: Bio-Rad Mini-Protean 3 Dodeca Cell. Update to EN 61010-1 Amendment Statement of Operating Conditions. Aviva Systems Biology is your western blotting protocol resource offering a wide range of primary. Instruction Manual, Criterion Blotter, Rev B. The system enables blot transfer of protein in as little as 3 minutes without sacrificing performance when compared with traditional tank protein blotting. Western Blotting Product Brochure, Rev C. Serves as a lab extension for the Comparative Proteomics I kit: Protein Profiling Duration Hands-on: Lab activity in four sessions, 45 min per session Comprehensive protein classroom study kit, includes Protein Profiler Module (166-2700EDU), Western Blot Module (166-2800EDU), Trans-Blot Turbo mini. The Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency. Features and Benefits: Application of Immunology Teaches students to use antibodies as detection tools. In this second part of the lab, students apply western blot techniques to their polyacrylamide gel results to specifically identify the myosin light chain among the hundreds of proteins that make up muscle cell extracts from near and distant fish species. 117179), students use sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to generate protein profiles and visualize the unique array of proteins that make up the muscle tissues of different fish. Proteins were then transferred to a 0,2m nitrocellulose membrane (Trans-Blot Turbo Transfer Pack, Bio-Rad, Cat 1704158) by using a western blot trans-blot. In the first part of this lab (Comparative Proteomics Kit I: Protein Profiling, ref. ![]() The "Comparative Proteomics II: Western Blot Module" kit is the second part of a two-part lab. ![]()
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